In this study, we synthesized Ag/Fe₃O₄ nanoparticles through the co-precipitation method, designating them as S1, S2, and S3 based on varying reaction times (RT) of 60, 30, and 15 minutes, respectively. The synthesized nanoparticles underwent microbiological tests and minimal inhibitory concentration (MIC) assays to assess their antibacterial potential against a spectrum of bacteria.  Building upon this, S2 exhibited superior antibacterial efficacy compared to both S1 and S3. Also, structural elucidation of Ag/Fe₃O₄ nanoparticles was achieved through a comprehensive analysis using UV-visible spectroscopy, a vibrating sample magnetometer (VSM), and Field Emission Scanning Electron Microscopy (FESEM). The morphological features, as determined by Field Emission Scanning Microscopy (FESM) revealed an average nanoparticle size of 25 nm. The engineered nanoparticles, especially highlighted by S2, show significant potential as potent antibacterial agents, particularly in scenarios where Gram-negative bacteria play a crucial role. This work integrates microbiological evaluations with structural considerations, contributing to a comprehensive understanding of these nanoparticles and paving the way for their potential applications in biomedical fields.

Keywords; Silver nanoparticles; antibacterial activity; co-precipitation, reaction time.