Cihan University-Erbil Conferences, 4th International Conference on Biological & Health Sciences (CIC-BIOHS’2022)

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Sequential Extractions of Secondary Metabolites Displaying Amylase Inhibitory Activity from Nutmeg (Myristica Fragrans) Mace
Ashok A Shinde, Faiyaz Khudaboddin Shaikh, Sarwan W. Bradosty

Last modified: 2022-08-30

Abstract


In the present investigation, the nutmeg mace is explored for secondary metabolites with amylase inhibitory activity that could be therapeutic agents to reduce hyperglycemia in diabetic complications. By using the sequential extraction method we have prepared methanol, ethyl acetate, acetone, hexane, and H2O extracts of nutmeg mace. These extracts were assessed for the presence of secondary metabolites. The qualitative analysis suggested the presence of terpenoids, flavonoids, and alkaloids in ethyl acetate extract. Upon quantitative estimation, acetone extract was found to contain 26 mg/ml GAE phenolics. The least amount of phenolics were found in hexane extract. Among all tested extracts, ethyl acetate showed strong inhibitory activity against salivary amylase on starch-agar plates. The salivary amylase inhibition potential of these extracts was further assessed by 7% SDS-PAGE incorporated with 0.1% starch. The phenolics present in ethyl acetate, methanol, and acetone extracts were displayed inhibition towards salivary amylase on the gel and could be further biochemically characterized for therapeutic applications to reduce hyperglycemia associated with diabetic complications.

Keywords-amylase, amylase inhibitors, secondary metabolites, hyperglycemia, diabetics.

 

DOI: http://doi.org/10.24086/biohs2022/paper.731


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