Cihan University-Erbil Conferences, 4th International Conference on Biological & Health Sciences (CIC-BIOHS’2022)

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FTIR spectrum analysis and antimicrobial activity of roots and leaves extracts of five Indian medicinal plants
wajed abdulla khan, Faiyaz Khudaboddin Shaikh, Sarwan W. Bradosty

Last modified: 2022-08-30

Abstract


The purpose of the present study was to find out the bioactive phytochemical content, their functional groups and antimicrobial activity of five selected Indian medicinal plants- Rauwolfia serpentine (roots), Tephrosia purpurae (leaves), Delonix regia (leaves), Withania somnifera (roots) and Asparagus racemosus (roots). The selected parts of plants were dried and the solvent extracts were prepared by distilled water extraction using Soxhlet apparatus. Phytochemicals were identified using primary screening test. Phytochemical analysis of plant extracts showed the presence of coumarins, steroids, saponins, phenolic compounds, flavonoids, alkaloids and sterols. FTIR analysis between the scan ranges of 400-4000 cm-1 is carried out. The FTIR spectroscopic investigation revealed that various functional groups like ethers, amines, phenol, ketone, carboxylic acids, alcohol, alkanes, aromatic compounds, etc. were identified. Antimicrobial investigations were done by using agar well diffusion method. It showed that the root extracts of Rauwolfia serpentina exhibit highest zone of inhibition against all test organisms- E. coli, S. aureus, S. typhi and Bacillus. From the results it is confirmed that these selected plants possess potential of bioactive compounds which are responsible for the biological activities that are useful for natural health.

Keywords- antimicrobial activity, medicinal plants, FTIR analysis, phenolic compounds, flavonoids, alkaloids.

 

DOI: http://doi.org/10.24086/biohs2022/paper.799


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